OligoAnalyzer Tool - Primer analysis and Tm Calculator IDT 1.0

OligoAnalyzer Tool – Primer Analysis and Tm Calculator IDT is a streamlined utility for designing and evaluating DNA and RNA oligonucleotides. It helps researchers and clinicians assess primer quality for PCR, qPCR, sequencing, cloning, and other molecular biology workflows by providing accurate thermodynamic calculations and structure predictions.

Key features:

• Melting temperature (Tm) calculation using nearest-neighbor thermodynamics with adjustable salt, Mg2+, dNTP, and oligo concentrations
• Quick metrics: GC content, length, molecular weight, and extinction coefficient
• Secondary structure analysis: hairpins, self-dimers, and hetero-dimers with ΔG estimates
• Primer pair assessment for compatibility, including 3' complementarity and potential primer-dimer formation
• Support for DNA, RNA, mixed/degenerate bases, and common modifications
• Convenient tools such as reverse complement, copy/paste input, and clean result summaries

Designed to reduce trial-and-error in primer design, this tool delivers fast, reliable insights so you can move from sequence to experiment with confidence.

OligoAnalyzer Tool - Primer analysis and Tm Calculator IDT is developed by Google\Chrome. The most popular version of this product among our users is 1.0.

You can check MixMeister BPM Analyzer, OLIGO, Oligo Analyzer and other related programs like A1 Website Analyzer at the "download" section.